Anti-NF-M Polyclonal antibody
Cat: K010019P
Summary:
| 【Product name】：Anti-NF-M Polyclonal antibody | 【Source】：Rabbit |
| 【Isotype】：IgG | 【Purification】：Affinity purification |
| 【Gene ID】：4741 | 【Swiss Prot】：P07197 |
| 【Observed】：140kDa | |
| 【Tested applications】：WB,IHC,ELISA,IF | |
| 【Recommended dilution】：WB 1:500-2000;IHC 1:50-500;IF 1:50-500. | |
| 【Immunogen】：Recombinant protein of human NF-M | |
| 【Public Immunogen Range】：1-97aa | |
| 【Storage】：Store at -20℃. Avoid freeze/thaw cycles. | |
Background:
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber. The names given to the three major neurofilament subunits are based upon the apparent molecular weight of the mammalian subunits on SDS-PAGE:NF-L, 65-68 kDa; NF-M,145-160 kDa and NF-H, 200-220 kDa. This antibody recognizes endogenous NF-M protein.
Verified picture:
Western blot analysis with NF-M antibody diluted at 1:1000;Lane: Mouse brain.
Immunohistochemistry of paraffin-embedded mouse cerebellum using NF-M antibody diluted at 1:200.
Immunofluorescence analysis of mouse brain using NF-M antibody diluted at 1:50.