Summary:
methyltransferase like 11A
Cat：P06436
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-224aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： METTL11A |
| 【Full Name】： methyltransferase like 11A |
| 【Uniprot】： Q9BV86 |
| 【Gene ID】： 28989 |
| 【Species Reactivity】： Human Mouse Rat (Bovine Zebrafish) |
| 【Mol Mass】： 25.4kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
METTL11A，also named N terminal RCC1 methyltransferase (NRMT)，is an alpha N methyltransferase that methylates the N terminal amino group of target proteins containing the N terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved (1).It is responsible for N terminal methylation of RCC1，KLHL31，MYL2，MYL3，RB1，RPL23A and SET.NRMT lacks a SET domain but possesses a Rossman like a/b fold.The residues Asn169，Asp178，Asp181，and Ser183 of NRMT are important for substrate binding (2).RCC1 (Ran guanine nucleotide exchange factor) is the first protein for which any biological function of alpha N methylation by NRMT has been identified (3，4).The multi spindle phenotype associated with either NRMT knockdown or methylation defective RCC1 mutants demonstrated the importance of alpha N methylation of RCC1 for normal bipolar spindle formation and chromosome aggregation (2).NRMT is robustly overexpressed in gastrointestinal cancers.
Verified picture
