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enoyl-Coenzyme A, hydratase/3-hydroxyacyl Coenzyme A dehydrogenase

Cat:P06525

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 350-700aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: EHHADH
【Full Name】: enoyl-Coenzyme A,hydratase/3-hydroxyacyl Coenzyme A dehydrogenase
【Uniprot】: Q08426
【Gene ID】: 1962
【Species Reactivity】: Human Mouse Rat (Zebrafish)
【Mol Mass】: 38.7kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

The protein encoded by this gene is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in this gene are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for this gene.

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