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galactose-3-O-sulfotransferase 1

Cat:P06854

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 46-395aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: GAL3ST1
【Full Name】: galactose-3-O-sulfotransferase 1
【Uniprot】: Q99999
【Gene ID】: 9514
【Mol Mass】: 40kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Catalyzes the transfer of a sulfate group to position 3 of non-reducing beta-galactosyl residues in glycerolipids and sphingolipids, therefore participates in the biosynthesis of sulfoglycolipids (PubMed:9030544, PubMed:8830034). Catalyzes the synthesis of galactosylceramide sulfate (sulfatide), a major lipid component of the myelin sheath and of monogalactosylalkylacylglycerol sulfate (seminolipid), present in spermatocytes (PubMed:8830034). Seems to prefer beta-glycosides at the non-reducing termini of sugar chains attached to a lipid moiety (PubMed:8830034). Also acts on lactosylceramide, galactosyl 1-alkyl-2-sn-glycerol and galactosyl diacylglycerol (in vitro) (PubMed:8830034).

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