下载

ATPase, H+ transporting, lysosomal V0 subunit a4

Cat：P06997

Summary:

【Derived From】： E.coli

【Endotoxin】： Not measured

【Amino Acid】： 89-430aa

【Purity】： ≥85% by SDS-PAGE.

【Name】： ATP6V0A4

【Full Name】： ATPase，H+ transporting，lysosomal V0 subunit a4

【Uniprot】： Q9HBG4

【Gene ID】： 50617

【Mol Mass】： 39kDa

【Application】： Immunology research

【Purification】： NI-NTA affinity purification

【Bioactive】： N0

【Tag】： With a 6×His tag at the N/C-terminus.

【Concentration】： 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)

【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year.

【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. This gene is one of four genes in man and mouse that encode different isoforms of the a subunit. Alternatively spliced transcript variants encoding the same protein have been described. Mutations in this gene are associated with renal tubular acidosis associated with preserved hearing.

Verified picture