Summary:
phospholipase C, zeta 1
Cat：P07683
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 60-340aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： PLCZ1 |
| 【Full Name】： phospholipase C，zeta 1 |
| 【Uniprot】： Q86YW0 |
| 【Gene ID】： 89869 |
| 【Mol Mass】： 31.8kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
The production of the second messenger molecules diacylglycerol (DAG) and inositol 1，4，5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes.In vitro，hydrolyzes PtdIns(4，5)P2 in a Ca2+-dependent manner.Triggers intracellular Ca2+ oscillations in oocytes solely during M phase and is involved in inducing oocyte activation and initiating embryonic development up to the blastocyst stage.Is therefore a strong candidate for the egg-activating soluble sperm factor that is transferred from the sperm into the egg cytoplasm following gamete membrane fusion.May exert an inhibitory effect on phospholipase-C-coupled processes that depend on calcium ions and protein kinase C，including CFTR trafficking and function.
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