Summary:
Recombinant human NSUN3 protein
Cat：P08043
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-300aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： NSUN3 |
| 【Full Name】： NOL1/NOP2/Sun domain family，member 3 |
| 【Uniprot】： Q9H649 |
| 【Gene ID】： 63899 |
| 【Species Reactivity】： Human Mouse (Bovine Zebrafish) |
| 【Mol Mass】： 34kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Mitochondrial tRNA methyltransferase that mediates methylation of cytosine to 5-methylcytosine (m5C at position 34 of mt-tRNA(Met. mt-tRNA(Met methylation at cytosine(34 takes place at the wobble position of the anticodon and initiates the formation of 5-formylcytosine (f(5c at this position. mt-tRNA(Met containing the f(5c modification at the wobble position enables recognition of the AUA codon in addition to the AUG codon, expanding codon recognition in mitochondrial translation.
Verified picture
