Summary:
Recombinant human ACCN2 protein
Cat：P08154
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 120-470AA |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： ACCN2 |
| 【Full Name】： amiloride-sensitive cation channel 2，neuronal |
| 【Uniprot】： P78348 |
| 【Gene ID】： 41 |
| 【Species Reactivity】： Human Mouse Rat (Chicken) |
| 【Mol Mass】： 40.1kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Isoform 2 and isoform 3 function as proton-gated sodium channels;they are activated by a drop of the extracellular pH and then become rapidly desensitized.The channel generates a biphasic current with a fast inactivating and a slow sustained phase.Has high selectivity for sodium ions and can also transport lithium ions with high efficiency.Isoform 2 can also transport potassium，but with lower efficiency.It is nearly impermeable to the larger rubidium and cesium ions.Isoform 3 can also transport calcium ions.Mediates glutamate-independent Ca2+ entry into neurons upon acidosis.This Ca2+ overloading is toxic for cortical neurons and may be in part responsible for ischemic brain injury.Heteromeric channel assembly seems to modulate channel properties.Functions as a postsynaptic proton receptor that influences intracellular Ca2+ concentration and calmodulin-dependent protein kinase II phosphorylation and thereby the density of dendritic spines.Modulates activity in the circuits underlying innate fear.Isoform 1 d
Verified picture
