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Recombinant human DCI protein

Cat:P02549

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-302aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: DCI
【Full Name】: dodecenoyl-Coenzyme A delta isomerase (3,2 trans-enoyl-Coenzyme A isomerase)
【Uniprot】: P42126
【Gene ID】: 1632
【Species Reactivity】: Human Mouse Rat (A.thaliana)
【Mol Mass】: 33kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

This gene encodes a member of the hydratase/isomerase superfamily.The protein encoded is a key mitochondrial enzyme involved in beta-oxidation of unsaturated fatty acids.It catalyzes the transformation of 3-cis and 3-trans-enoyl-CoA esters arising during the stepwise degradation of cis-, mono-, and polyunsaturated fatty acids to the 2-trans-enoyl-CoA intermediates.Alternatively spliced transcript variants have been described.

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