Summary:
Recombinant human MCM10 protein
Cat：P02551
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 520-875aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： MCM10 |
| 【Full Name】： minichromosome maintenance complex component 10 |
| 【Uniprot】： Q7L590 |
| 【Gene ID】： 55388 |
| 【Species Reactivity】： Human Mouse (Zebrafish Fruit fly) |
| 【Mol Mass】： 40kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication.The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre-RC) and it may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins.This protein can interact with MCM2 and MCM6, as well as with the origin recognition protein ORC2.It is regulated by proteolysis and phosphorylation in a cell cycle-dependent manner.Studies of a similar protein in Xenopus suggest that the chromatin binding of this protein at the onset of DNA replication is after pre-RC assembly and before origin unwinding.Alternatively spliced transcript variants encoding distinct isoforms have been identified
Verified picture
