Summary:
Recombinant human DFFA protein
Cat：P02607
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-331aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： DFFA |
| 【Full Name】： DNA fragmentation factor，45kDa，alpha polypeptide |
| 【Uniprot】： O00273 |
| 【Gene ID】： 1676 |
| 【Species Reactivity】： Human Mouse |
| 【Mol Mass】： 37kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development.The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units.DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits.DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis.DFF becomes activated when DFFA is cleaved by caspase-3.The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF.DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis.Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
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