Summary:
Recombinant human GNB3 protein
Cat：P02682
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-340aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： GNB3 |
| 【Full Name】： guanine nucleotide binding protein (G protein)，beta polypeptide 3 |
| 【Uniprot】： P16520 |
| 【Gene ID】： 2784 |
| 【Species Reactivity】： Human Mouse Rat (Dog) |
| 【Mol Mass】： 37kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Heterotrimeric guanine nucleotide-binding proteins (G proteins), which integrate signals between receptors and effector proteins, are composed of an alpha, a beta, and a gamma subunit.These subunits are encoded by families of related genes.This gene encodes a beta subunit which belongs to the WD repeat G protein beta family.Beta subunits are important regulators of alpha subunits, as well as of certain signal transduction receptors and effectors.A single-nucleotide polymorphism (C825T) in this gene is associated with essential hypertension and obesity.This polymorphism is also associated with the occurrence of the splice variant GNB3-s, which appears to have increased activity.GNB3-s is an example of alternative splicing caused by a nucleotide change outside of the splice donor and acceptor sites.Alternative splicing results in multiple transcript variants.Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known.
Verified picture
