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Recombinant human PRIM1 protein

Cat:P02701

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-420aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: PRIM1
【Full Name】: DNA primase small subunit
【Uniprot】: P49642
【Gene ID】: 5557
【Species Reactivity】: Human Mouse
【Mol Mass】: 50kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components.Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication.The protein encoded by this gene is the small, 49 kDa primase subunit.

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