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Recombinant human BLMH protein

Cat:P02754

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 105-455aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: BLMH
【Full Name】: bleomycin hydrolase
【Uniprot】: Q13867
【Gene ID】: 642
【Species Reactivity】: Human Mouse Rat (Rabbit Chicken)
【Mol Mass】: 40kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Bleomycin hydrolase (BMH) is a cytoplasmic cysteine peptidase that is highly conserved through evolution; however, the only known activity of the enzyme is metabolic inactivation of the glycopeptide bleomycin (BLM), an essential component of combination chemotherapy regimens for cancer.The protein contains the signature active site residues of the cysteine protease papain superfamily.

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