Summary:
Recombinant human MIEF1 protein
Cat：P02771
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-350aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： MIEF1 |
| 【Full Name】： Mitochondrial dynamics protein MID51 |
| 【Uniprot】： Q9NQG6 |
| 【Gene ID】： 54471 |
| 【Species Reactivity】： Human Mouse Rat (Zebrafish Gorilla) |
| 【Mol Mass】： 39kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Mitochondrial outer membrane protein which regulates mitochondrial fission.Promotes the recruitment and association of the fission mediator dynamin-related protein 1 (DNM1L) to the mitochondrial surface independently of the mitochondrial fission FIS1 and MFF proteins.Regulates DNM1L GTPase activity and DNM1L oligomerization.Binds ADP and can also bind GDP, although with lower affinity.Does not bind CDP, UDP, ATP, AMP or GTP.Inhibits DNM1L GTPase activity in the absence of bound ADP.Requires ADP to stimulate DNM1L GTPase activity and the assembly of DNM1L into long, oligomeric tubules with a spiral pattern, as opposed to the ring-like DNM1L oligomers observed in the absence of bound ADP.Does not require ADP for its function in recruiting DNM1L.
Verified picture
