Summary:
Recombinant human SF1 protein
Cat：P02798
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-310aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： SF1 |
| 【Full Name】： splicing factor 1 |
| 【Uniprot】： Q15637 |
| 【Gene ID】： 7536 |
| 【Species Reactivity】： Human Mouse Rat (Bovine Gorilla) |
| 【Mol Mass】： 35kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
This gene encodes a nuclear pre-mRNA splicing factor.The encoded protein specifically recognizes the intron branch point sequence at the 3' splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly.It also plays a role in nuclear pre-mRNA retention and transcriptional repression.The encoded protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region which bind a key branch-site adenosine within the branch point sequence, a zinc knuckles domain, and a C-terminal proline-rich domain.Alternative splicing results in multiple transcript variants.May act as transcription repressor.
Verified picture
