Summary:
Recombinant human MRPL53 protein
Cat：P02871
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-112aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： MRPL53 |
| 【Full Name】： mitochondrial ribosomal protein L53 |
| 【Uniprot】： Q96EL3 |
| 【Gene ID】： 116540 |
| 【Species Reactivity】： Human Mouse (Bovine Gorilla) |
| 【Mol Mass】： 12kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion.Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit.They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed.Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA.Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.This gene encodes a 39S subunit protein.A pseudogene corresponding to this gene is found on chromosome 1p.
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