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Recombinant human ATP5J protein

Cat:P02872

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-108aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: ATP5J
【Full Name】: ATP synthase-coupling factor 6,mitochondrial
【Uniprot】: P18859
【Gene ID】: 522
【Species Reactivity】: Human Mouse Rat (Bovine Monkey Pig Gorilla)
【Mol Mass】: 13kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation.It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel.The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3.The Fo complex has nine subunits (a, b, c, d, e, f, g, F6 and 8).This gene encodes the F6 subunit of the Fo complex.The F6 subunit is required for F1 and Fo interactions.Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.This gene has 1 or more pseudogenes.

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