Summary:
Recombinant human LIG4 protein
Cat：P02902
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 562-911aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： LIG4 |
| 【Full Name】： DNA ligase 4 |
| 【Uniprot】： P49917 |
| 【Gene ID】： 3981 |
| 【Species Reactivity】： Human Mouse (Chicken) |
| 【Mol Mass】： 38kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
The protein encoded by this gene is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction.This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ).This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK).Both XRCC4 and DNA-PK are known to be required for NHEJ.The crystal structure of the complex formed by this protein and XRCC4 has been resolved.Defects in this gene are the cause of LIG4 syndrome.Alternatively spliced transcript variants encoding the same protein have been observed.
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