Summary:
Recombinant human PLOD2 protein
Cat：P02949
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-350aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： PLOD2 |
| 【Full Name】： procollagen-lysine，2-oxoglutarate 5-dioxygenase 2 |
| 【Uniprot】： O00469 |
| 【Gene ID】： 5352 |
| 【Species Reactivity】： Human Mouse Rat |
| 【Mol Mass】： 40kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum.The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides.The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks.Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity.Mutations in the coding region of this gene are associated with Bruck syndrome.Alternative splicing results in multiple transcript variants encoding different isoforms.
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