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Recombinant human NDUFAF1 protein

Cat:P03010

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-327aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: NDUFAF1
【Full Name】: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex,assembly factor 1
【Uniprot】: Q9Y375
【Gene ID】: 51103
【Species Reactivity】: Human Mouse (Gorilla)
【Mol Mass】: 38kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

This gene encodes a complex I assembly factor protein.Complex I (NADH-ubiquinone oxidoreductase) catalyzes the transfer of electrons from NADH to ubiquinone (coenzyme Q) in the first step of the mitochondrial respiratory chain, resulting in the translocation of protons across the inner mitochondrial membrane.The encoded protein is required for assembly of complex I, and mutations in this gene are a cause of mitochondrial complex I deficiency.Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 19.

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