Summary:
Recombinant human METTL14 protein
Cat：P03035
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 159-456aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： METTL14 |
| 【Full Name】： N6-adenosine-methyltransferase non-catalytic subunit |
| 【Uniprot】： Q9HCE5 |
| 【Gene ID】： 57721 |
| 【Species Reactivity】： Human Mouse |
| 【Mol Mass】： 33kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
The METTL3-METTL14 heterodimer forms a N6-methyltransferase complex that methylates adenosine residues at the N6 position of some mRNAs and regulates the circadian clock, differentiation of embryonic stem cells and cortical neurogenesis .In the heterodimer formed with METTL3, METTL14 constitutes the RNA-binding scaffold that recognizes the substrate rather than the catalytic core .N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in mRNA stability and processing M6A acts as a key regulator of mRNA stability by promoting mRNA destabilization and degradation (By similarity).In embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization (By similarity).M6A regulates spermatogonial differentiation and meiosis and is essential for male fertility and spermatogenesis (By similarity).M6A also regulates cortical neurogenesis: m6A methylation of transcripts related to transcr
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