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Recombinant human DARS protein

Cat:P03056

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-350aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: DARS
【Full Name】: aspartyl-tRNA synthetase
【Uniprot】: P14868
【Gene ID】: 1615
【Species Reactivity】: Human Mouse Rat (Bovine)
【Mol Mass】: 40kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

This gene encodes a member of a multienzyme complex that functions in mediating the attachment of amino acids to their cognate tRNAs.The encoded protein ligates L-aspartate to tRNA(Asp).Mutations in this gene have been found in patients showing hypomyelination with brainstem and spinal cord involvement and leg spasticity.Alternative splicing results in multiple transcript variants.

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