Summary:
Recombinant human H3F3B protein
Cat：P03071
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-136aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： H3F3B |
| 【Full Name】： Histone H3.3 |
| 【Uniprot】： P84243 |
| 【Gene ID】： 3021 |
| 【Species Reactivity】： Human Mouse Rat (Bovine Zebrafish) |
| 【Mol Mass】： 15kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes.Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes.The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures.This gene contains introns and its mRNA is polyadenylated, unlike most histone genes.The protein encoded by this gene is a replication-independent histone that is a member of the histone H3 family.Pseudogenes of this gene have been identified on the X chromosome, and on chromosomes 5, 13 and 17.
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