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Recombinant human TAF12 protein

Cat：P03133

Summary:

【Derived From】： E.coli

【Endotoxin】： Not measured

【Amino Acid】： 1-161aa

【Purity】： ≥85% by SDS-PAGE.

【Name】： TAF12

【Full Name】： Transcription initiation factor TFIID subunit 12

【Uniprot】： Q16514

【Gene ID】： 6883

【Species Reactivity】： Human Mouse (Bovine Fruit fly)

【Mol Mass】： 18kDa

【Application】： Immunology research

【Purification】： NI-NTA affinity purification

【Bioactive】： N0

【Tag】： With a 6×His tag at the N/C-terminus.

【Concentration】： 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)

【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year.

【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Control of transcription by RNA polymerase II involves the basal transcription machinery which is a collection of proteins.These proteins with RNA polymerase II, assemble into complexes which are modulated by transactivator proteins that bind to cis-regulatory elements located adjacent to the transcription start site.Some modulators interact directly with the basal complex, whereas others may act as bridging proteins linking transactivators to the basal transcription factors.Some of these associated factors are weakly attached while others are tightly associated with TBP in the TFIID complex.Among the latter are the TAF proteins.Different TAFs are predicted to mediate the function of distinct transcriptional activators for a variety of gene promoters and RNA polymerases.TAF12 interacts directly with TBP as well as with TAF2I.Two transcript variants encoding the same protein have been found for this gene.

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