Summary:
Recombinant human SPIN2B protein
Cat：P03197
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-259aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： SPIN2B |
| 【Full Name】： spindlin family，member 2B |
| 【Uniprot】： Q9BPZ2 |
| 【Gene ID】： 474343 |
| 【Species Reactivity】： Human Rat |
| 【Mol Mass】： 29kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Human SPIN2 expressed by 2 IL3-dependent murine myeloid cell lines was moderately antiapoptotic following IL3 removal, but it was unable to block apoptosis induced by the chemotherapy agent doxorubicin.On the contrary, SPIN2-expressing cells were more sensitive to doxorubicin-mediated cell death.Also, SPIN2 did not combat the proapoptotic effects of Fas ligand stimulation in the Jurkat human T-cell line.Deletion of the C-terminal amino acids of SPIN2 diminished its antiapoptotic activity.In the murine myeloid cells, SPIN2 overexpression increased the cell number doubling times and slowed their rate of growth.There was also an increased percentage of cells in G2/M, which was more pronounced following IL3 withdrawal.
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