Summary:
Recombinant human TRPM8 protein
Cat：P03218
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-192aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： TRPM8 |
| 【Full Name】： transient receptor potential cation channel，subfamily M，member 8 |
| 【Uniprot】： Q7Z2W7 |
| 【Gene ID】： 79054 |
| 【Species Reactivity】： Human Mouse Rat |
| 【Mol Mass】： 23kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius.Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH.Involved in menthol sensation.Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.Temperature sensing is tightly linked to voltage-dependent gating.Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves.The chemical agonist menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials.Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.In prostate cancer cells, shows strong inward rectification and high calcium selectivity in contrast to its behavior in normal cells which is characterized by outward rectification and poor cationi
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