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Recombinant human EIF3K protein

Cat:P03258

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-218aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: EIF3K
【Full Name】: eukaryotic translation initiation factor 3,subunit K
【Uniprot】: Q9UBQ5
【Gene ID】: 27335
【Species Reactivity】: Human Mouse (Bovine Zebrafish Fruit fly)
【Mol Mass】: 25kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

The 700-kD eukaryotic translation initiation factor-3 (eIF3) is the largest eIF and contains at least 12 subunits, including EIF2S12.eIF3 plays an essential role in translation by binding directly to the 40S ribosomal subunit and promoting formation of the 40S preinitiation complex (Mayeur et al., 2003 [PubMed 14519125]).

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