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Recombinant human UBE2E3 protein

Cat:P03259

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-207aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: UBE2E3
【Full Name】: ubiquitin-conjugating enzyme E2E 3 (UBC4/5 homolog,yeast)
【Uniprot】: Q969T4
【Gene ID】: 10477
【Species Reactivity】: Human Mouse (Bovine)
【Mol Mass】: 23kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: No activity experimental data, to be verified.
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 2-7 days, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.This gene encodes a member of the E2 ubiquitin-conjugating enzyme family.The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes.Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene.

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