下载

Recombinant human IDH1 protein

Cat:P04353

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-414aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: IDH1
【Full Name】: isocitrate dehydrogenase 1 (NADP+),soluble
【Uniprot】: O75874
【Gene ID】: 3417
【Species Reactivity】: Human Mouse Rat (Bovine Pig Sheep Orangutan)
【Mol Mass】: 47kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Isocitrate deHumanydrogenases catalyze tHumane oxidative decarboxylation of isocitrate to 2-oxoglutarate. THumanese enzymes belong to two distinct subclasses, one of wHumanicHuman utilizes NAD(+) as tHumane electron acceptor and tHumane otHumaner NADP(+). Five isocitrate deHumanydrogenases Humanave been reported: tHumanree NAD(+)-dependent isocitrate deHumanydrogenases, wHumanicHuman localize to tHumane mitocHumanondrial matrix, and two NADP(+)-dependent isocitrate deHumanydrogenases, one of wHumanicHuman is mitocHumanondrial and tHumane otHumaner predominantly cytosolic. EacHuman NADP(+)-dependent isozyme is a Humanomodimer. THumane protein encoded by tHumanis gene is tHumane NADP(+)-dependent isocitrate deHumanydrogenase found in tHumane cytoplasm and peroxisomes. It contains tHumane PTS-1 peroxisomal targeting signal sequence. THumane presence of tHumanis enzyme in peroxisomes suggests roles in tHumane regeneration of NADPHuman for intraperoxisomal reductions, sucHuman as tHumane conversion of 2, 4-dienoyl

Verified picture