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Recombinant human SET protein

Cat：P04721

Summary:

【Derived From】： E.coli

【Endotoxin】： Not measured

【Amino Acid】： 1-277aa

【Purity】： ≥85% by SDS-PAGE.

【Name】： SET

【Full Name】： SET nuclear oncogene

【Uniprot】： Q01105

【Gene ID】： 6418

【Species Reactivity】： Human (Fruit fly)

【Mol Mass】： 33kDa

【Application】： Immunology research

【Purification】： NI-NTA affinity purification

【Bioactive】： No activity experimental data, to be verified.

【Tag】： With a 6×His tag at the N/C-terminus.

【Concentration】： 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.)

【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 2-7 days， stored at < -20℃ for 1 year.

【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

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