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Recombinant human BRF1 protein

Cat:P04842

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-208aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: BRF1
【Full Name】: BRF1 homolog,subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)
【Uniprot】: Q92994
【Gene ID】: 2972
【Species Reactivity】: Human Mouse Rat
【Mol Mass】: 22kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

General activator of RNA polymerase which utilizes different TFIIIB complexes at structurally distinct promoters. The isoform 1 is involved in the transcription of tRNA, adenovirus VA1, 7SL and 5S RNA. Isoform 2 is required for transcription of the U6 promoter.

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