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Recombinant human UBE2E2 protein

Cat:P05463

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-202aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: UBE2E2
【Full Name】: ubiquitin-conjugating enzyme E2E 2 (UBC4/5 homolog,yeast)
【Uniprot】: Q96LR5
【Gene ID】: 7325
【Species Reactivity】: Human Mouse
【Mol Mass】: 22kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: No activity experimental data, to be verified.
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 2-7 days, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination. Catalyzes the ISGylation of influenza A virus NS1 protein.

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