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Recombinant human ATP5H protein

Cat:P03485

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-161aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: ATP5H
【Full Name】: ATP synthase,H+ transporting,mitochondrial F0 complex,subunit d
【Uniprot】: O75947
【Gene ID】: 10476
【Species Reactivity】: Human Mouse Rat (Bovine)
【Mol Mass】: 18kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation.It is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, which comprises the proton channel.The F1 complex consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled in a ratio of 3 alpha, 3 beta, and a single representative of the other 3.The Fo seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8).This gene encodes the d subunit of the Fo complex.Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.In addition, three pseudogenes are located on chromosomes 9, 12 and 15.

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