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Recombinant human CKMT2 protein

Cat：P03525

Summary:

【Derived From】： E.coli

【Endotoxin】： Not measured

【Amino Acid】： 1-419aa

【Purity】： ≥85% by SDS-PAGE.

【Name】： CKMT2

【Full Name】： creatine kinase，mitochondrial 2 (sarcomeric)

【Uniprot】： P17540

【Gene ID】： 1160

【Species Reactivity】： Human (Bovine Mouse Rat Chicken Rabbit Gorilla)

【Mol Mass】： 48kDa

【Application】： Immunology research

【Purification】： NI-NTA affinity purification

【Bioactive】： N0

【Tag】： With a 6×His tag at the N/C-terminus.

【Concentration】： 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)

【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year.

【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Mitochondrial creatine kinase (MtCK) is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine.It belongs to the creatine kinase isoenzyme family.It exists as two isoenzymes, sarcomeric MtCK and ubiquitous MtCK, encoded by separate genes.Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes.Sarcomeric mitochondrial creatine kinase has 80% homology with the coding exons of ubiquitous mitochondrial creatine kinase.This gene contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins and thus may be essential for the coordinated activation of these genes during mitochondrial biogenesis.Three transcript variants encoding the same protein have been found for this gene.

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