Summary:
Recombinant human FH protein
Cat：P03540
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 161-510aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： FH |
| 【Full Name】： fumarate hydratase |
| 【Uniprot】： P07954 |
| 【Gene ID】： 2271 |
| 【Species Reactivity】： Human Mouse Rat (Fruit fly Pig Bovine Zebrafish A.thaliana Monkey) |
| 【Mol Mass】： 39kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
The protein encoded by this gene is an enzymatic component of the tricarboxylic acid (TCA) cycle, or Krebs cycle, and catalyzes the formation of L-malate from fumarate.It exists in both a cytosolic form and an N-terminal extended form, differing only in the translation start site used.The N-terminal extended form is targeted to the mitochondrion, where the removal of the extension generates the same form as in the cytoplasm.It is similar to some thermostable class II fumarases and functions as a homotetramer.Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy.
Verified picture
