Summary:
Recombinant human CDC42 protein
Cat：P03603
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-191aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： CDC42 |
| 【Full Name】： cell division cycle 42 (GTP binding protein，25kDa) |
| 【Uniprot】： P60953 |
| 【Gene ID】： 998 |
| 【Species Reactivity】： Human Mouse Rat (Bovine Pig Fruit fly Chicken) |
| 【Mol Mass】： 21kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression.This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant.The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein.This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex.Alternative splicing of this gene results in multiple transcript variants.Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20
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