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Recombinant human TAF9B protein

Cat:P03616

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-251aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: TAF9B
【Full Name】: TAF9B RNA polymerase II,TATA box binding protein (TBP)-associated factor,31kDa
【Uniprot】: Q9HBM6
【Gene ID】: 51616
【Species Reactivity】: Human Mouse Rat (Gorilla)
【Mol Mass】: 28kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Initiation of transcription by RNA polymerase II requires the activities of more than 70 polypeptides.The protein that coordinates these activities is transcription factor IID (TFIID), which binds to the core promoter to position the polymerase properly, serves as the scaffold for assembly of the remainder of the transcription complex, and acts as a channel for regulatory signals.TFIID is composed of the TATA-binding protein (TBP) and a group of evolutionarily conserved proteins known as TBP-associated factors or TAFs.TAFs may participate in basal transcription, serve as coactivators, function in promoter recognition or modify general transcription factors (GTFs) to facilitate complex assembly and transcription initiation.This gene encodes a protein that is similar to one of the small subunits of TFIID, TBP-associated factor 9, and is also a subunit of TFIID.TAF9 and TAF9b share some functions but also have distinct roles in the transcriptional regulatory process.

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