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Recombinant human UBE2H protein

Cat:P03620

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-183aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: UBE2H
【Full Name】: ubiquitin-conjugating enzyme E2H (UBC8 homolog,yeast)
【Uniprot】: P62256
【Gene ID】: 7328
【Species Reactivity】: Human Mouse (Bovine)
【Mol Mass】: 21kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: No activity experimental data, to be verified.
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 10μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 2-7 days, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation.Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s.This gene encodes a member of the E2 ubiquitin-conjugating enzyme family.The encoded protein sequence is 100% identical to the mouse homolog and 98% identical to the frog and zebrafish homologs.Three alternatively spliced transcript variants have been found for this gene and they encode distinct isoforms.

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