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Recombinant human CHMP4B protein

Cat：P03623

Summary:

【Derived From】： E.coli

【Endotoxin】： Not measured

【Amino Acid】： 1-224aa

【Purity】： ≥85% by SDS-PAGE.

【Name】： CHMP4B

【Full Name】： chromatin modifying protein 4B

【Uniprot】： Q9H444

【Gene ID】： 128866

【Species Reactivity】： Human Mouse (Chicken Zebrafish)

【Mol Mass】： 25kDa

【Application】： Immunology research

【Purification】： NI-NTA affinity purification

【Bioactive】： N0

【Tag】： With a 6×His tag at the N/C-terminus.

【Concentration】： 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)

【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year.

【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

This gene encodes a member of the chromatin-modifying protein/charged multivesicular body protein (CHMP) protein family.The protein is part of the endosomal sorting complex required for transport (ESCRT) complex III (ESCRT-III), which functions in the sorting of endocytosed cell-surface receptors into multivesicular endosomes.The ESCRT machinery also functions in the final abscisson stage of cytokinesis and in the budding of enveloped viruses such as HIV-1.The three proteins of the CHMP4 subfamily interact with programmed cell death 6 interacting protein (PDCD6IP, also known as ALIX), which also functions in the ESCRT pathway.The CHMP4 proteins assemble into membrane-attached 5-nm filaments that form circular scaffolds and promote or stabilize outward budding.These polymers are proposed to help generate the luminal vesicles of multivesicular bodies.Mutations in this gene result in autosomal dominant posterior polar cataracts.

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