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Recombinant human PUF60 protein

Cat:P03669

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 197-542aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: PUF60
【Full Name】: poly-U binding splicing factor 60KDa
【Uniprot】: Q9UHX1
【Gene ID】: 22827
【Species Reactivity】: Human Mouse Rat (Bovine Gorilla Zebrafish)
【Mol Mass】: 38kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

This gene encodes a nucleic acid-binding protein that plays a role in a variety of nuclear processes, including pre-mRNA splicing and transcriptional regulation.The encoded protein forms a complex with the far upstream DNA element (FUSE) and FUSE-binding protein at the myelocytomatosis oncogene (MYC) promoter.This complex represses MYC transcription through the core-TFIIH basal transcription factor.Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

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