Summary:
Recombinant human UBE2F protein
Cat：P03685
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-185aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： UBE2F |
| 【Full Name】： ubiquitin-conjugating enzyme E2F (putative) |
| 【Uniprot】： Q969M7 |
| 【Gene ID】： 140739 |
| 【Species Reactivity】： Human Mouse Rat (Bovine Chicken Zebrafish) |
| 【Mol Mass】： 21kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
The protein encoded by this gene is a member of the E2F family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses.The E2F proteins contain several evolutionally conserved domains found in most members of the family.These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain.This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain.This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner.It can mediate both cell proliferation and p53-dependent/independent apoptosis.
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