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Recombinant human NFS1 protein

Cat:P03704

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-190aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: NFS1
【Full Name】: NFS1 nitrogen fixation 1 homolog (S. cerevisiae)
【Uniprot】: Q9Y697
【Gene ID】: 9054
【Species Reactivity】: Human Mouse Rat (Fruit fly Gorilla)
【Mol Mass】: 21kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Iron-sulfur clusters are required for the function of many cellular enzymes.The proteins encoded by this gene supply inorganic sulfur to these clusters by removing the sulfur from cysteine, creating alanine in the process.This gene uses alternate in-frame translation initiation sites to generate mitochondrial forms and cytoplasmic/nuclear forms.Selection of the alternative initiation sites is determined by the cytosolic pH.The encoded proteins belong to the class-V family of pyridoxal phosphate-dependent aminotransferases.Alternatively spliced transcript variants have been described.

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