Summary:
Recombinant human HLA-DOB protein
Cat：P03724
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-273aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： HLA-DOB |
| 【Full Name】： major histocompatibility complex，class II，DO beta |
| 【Uniprot】： P13765 |
| 【Gene ID】： 3112 |
| 【Species Reactivity】： Human |
| 【Mol Mass】： 31kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
HLA-DOB belongs to the HLA class II beta chain paralogues.This class II molecule is a heterodimer consisting of an alpha (DOA) and a beta chain (DOB), both anchored in the membrane.It is located in intracellular vesicles.DO suppresses peptide loading of MHC class II molecules by inhibiting HLA-DM.Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages).The beta chain is approximately 26-28 kDa and its gene contains 6 exons.Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
Verified picture
