Summary:
Recombinant human CNOT8 protein
Cat：P03748
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-292aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： CNOT8 |
| 【Full Name】： CCR4-NOT transcription complex，subunit 8 |
| 【Uniprot】： Q9UFF9 |
| 【Gene ID】： 9337 |
| 【Species Reactivity】： Human Mouse |
| 【Mol Mass】： 34kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Has 3'-5' poly(A) exoribonuclease activity for synthetic poly(A) RNA substrate.Its function seems to be partially redundant with that of CNOT7.Catalytic component of the CCR4-NOT complex which is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation.During miRNA-mediated repression the complex seems also to act as translational repressor during translational initiation.Additional complex functions may be a consequence of its influence on mRNA expression.Associates with members of the BTG family such as TOB1 and BTG2 and is required for their anti-proliferative activity.
Verified picture
