Summary:
Recombinant human RAB3A protein
Cat：P03759
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-220aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： RAB3A |
| 【Full Name】： RAB3A，member RAS oncogene family |
| 【Uniprot】： P20336 |
| 【Gene ID】： 5864 |
| 【Species Reactivity】： Human Mouse Rat (Pig Bovine) |
| 【Mol Mass】： 25kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Small GTP-binding protein that plays a central role in regulated exocytosis and secretion.Controls the recruitment, tethering and docking of secretory vesicles to the plasma membrane.Upon stimulation, switches to its active GTP-bound form, cycles to vesicles and recruits effectors such as RIMS1, RIMS2, Rabphilin-3A/RPH3A, RPH3AL or SYTL4 to help the docking of vesicules onto the plasma membrane.Upon GTP hydrolysis by GTPase-activating protein, dissociates from the vesicle membrane allowing the exocytosis to proceed.Stimulates insulin secretion through interaction with RIMS2 or RPH3AL effectors in pancreatic beta cells.Regulates calcium-dependent lysosome exocytosis and plasma membrane repair (PMR) via the interaction with 2 effectors, SYTL4 and myosin-9/MYH9.Acts as a positive regulator of acrosome content secretion in sperm cells by interacting with RIMS1.Plays also a role in the regulation of dopamine release by interacting with synaptotagmin I/SYT.
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