Summary:
Recombinant human SFRS1 protein
Cat：P03774
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-248aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： SFRS1 |
| 【Full Name】： splicing factor，arginine/serine-rich 1 |
| 【Uniprot】： Q07955 |
| 【Gene ID】： 6426 |
| 【Species Reactivity】： Human Mouse (Bovine Pig Gorilla Chicken) |
| 【Mol Mass】： 28kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Plays a role in preventing exon skipping, ensuring the accuracy of splicing and regulating alternative splicing.Interacts with other spliceosomal components, via the RS domains, to form a bridge between the 5'- and 3'-splice site binding components, U1 snRNP and U2AF.Can stimulate binding of U1 snRNP to a 5'-splice site-containing pre-mRNA.Binds to purine-rich RNA sequences, either the octamer, 5'-RGAAGAAC-3' (r=A or G) or the decamers, AGGACAGAGC/AGGACGAAGC.Binds preferentially to the 5'-CGAGGCG-3' motif in vitro.Three copies of the octamer constitute a powerful splicing enhancer in vitro, the ASF/SF2 splicing enhancer (ASE) which can specifically activate ASE-dependent splicing.Isoform ASF-2 and isoform ASF-3 act as splicing repressors.May function as export adapter involved in mRNA nuclear export through the TAP/NXF1 pathway.
Verified picture
