Summary:
Recombinant human WAC protein
Cat：P03936
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-210aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： WAC |
| 【Full Name】： WW domain containing adaptor with coiled-coil |
| 【Uniprot】： Q9BTA9 |
| 【Gene ID】： 51322 |
| 【Species Reactivity】： Human Mouse |
| 【Mol Mass】： 23kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
Acts as a linker between gene transcription and histone H2B monoubiquitination at 'Lys-120' (H2BK120ub1) ref.Interacts with the RNA polymerase II transcriptional machinery via its WW domain and with RNF20-RNF40 via its coiled coil region, thereby linking and regulating H2BK120ub1 and gene transcription ref.Regulates the cell-cycle checkpoint activation in response to DNA damage ref.Positive regulator of amino acid starvation-induced autophagy ref.Also acts as a negative regulator of basal autophagy ref.Positively regulates MTOR activity by promoting, in an energy-dependent manner, the assembly of the TTT complex composed of TELO2, TTI1 and TTI2 and the RUVBL complex composed of RUVBL1 and RUVBL2 into the TTT-RUVBL complex.This leads to the dimerization of the mTORC1 complex and its subsequent activation ref.May negatively regulate the ubiquitin proteasome pathway ref.
Verified picture
