Summary:
Recombinant human ECH1 protein
Cat：P03975
| 【Derived From】： E.coli |
| 【Endotoxin】： Not measured |
| 【Amino Acid】： 1-329aa |
| 【Purity】： ≥85% by SDS-PAGE. |
| 【Name】： ECH1 |
| 【Full Name】： enoyl Coenzyme A hydratase 1，peroxisomal |
| 【Uniprot】： Q13011 |
| 【Gene ID】： 1891 |
| 【Species Reactivity】： Human Mouse Rat (Gorilla) |
| 【Mol Mass】： 36kDa |
| 【Application】： Immunology research |
| 【Purification】： NI-NTA affinity purification |
| 【Bioactive】： N0 |
| 【Tag】： With a 6×His tag at the N/C-terminus. |
| 【Concentration】： 1mg/ml by SDS-PAGE. |
Store:
| 【Reconstitution】： Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.) |
| 【Storage】： Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks， stored at < -20℃ for 1 year. |
| 【Formulation】： Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0. |
Background:
This gene encodes a member of the hydratase/isomerase superfamily.The gene product shows high sequence similarity to enoyl-coenzyme A (CoA) hydratases of several species, particularly within a conserved domain characteristic of these proteins.The encoded protein, which contains a C-terminal peroxisomal targeting sequence, localizes to the peroxisome.The rat ortholog, which localizes to the matrix of both the peroxisome and mitochondria, can isomerize 3-trans,5-cis-dienoyl-CoA to 2-trans,4-trans-dienoyl-CoA, indicating that it is a delta3,5-delta2,4-dienoyl-CoA isomerase.This enzyme functions in the auxiliary step of the fatty acid beta-oxidation pathway.Expression of the rat gene is induced by peroxisome proliferators.
Verified picture
