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Recombinant human RNPC3 protein

Cat:P04029

Summary:

【Derived From】: E.coli
【Endotoxin】: Not measured
【Amino Acid】: 1-262aa
【Purity】: ≥85% by SDS-PAGE.
【Name】: RNPC3
【Full Name】: RNA-binding region (RNP1,RRM) containing 3
【Uniprot】: Q96LT9
【Gene ID】: 55599
【Species Reactivity】: Human Mouse Rat (Bovine Gorilla)
【Mol Mass】: 30kDa
【Application】: Immunology research
【Purification】: NI-NTA affinity purification
【Bioactive】: N0
【Tag】: With a 6×His tag at the N/C-terminus.
【Concentration】: 1mg/ml by SDS-PAGE.

Store:

【Reconstitution】: Reconstituted protein solution can be diluted with distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. (It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water.)
【Storage】: Reconstituted protein solution can be stored at 4-7℃ for 1-2 weeks, stored at < -20℃ for 1 year.
【Formulation】: Powder: Lyophilized from a 0.2 μm filtered solution of 2-8M Urea, 20mM Tris-HCl, 150mM NaCl, 1mM DTT, PH7.2-8.0.

Background:

Two types of spliceosomes catalyze splicing of pre-mRNAs.The major U2-type spliceosome is found in all eukaryotes and removes U2-type introns, which represent more than 99% of pre-mRNA introns.The minor U12-type spliceosome is found in some eukaryotes and removes U12-type introns, which are rare and have distinct splice consensus signals.The U12-type spliceosome consists of several small nuclear RNAs and associated proteins.This gene encodes a 65K protein that is a component of the U12-type spliceosome.This protein contains two RNA recognition motifs (RRMs), suggesting that it may contact one of the small nuclear RNAs of the minor spliceosome.

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